Menu in Datalyse:
Measure at one wavelength
Spectrum, wavelength scan
You can also use the terminal program in Datalyse with the spectrophotometer.
The LBK Ultrospec II Spectrophotometer is connected to the pc with an ordinary serial cable. (same type as for the IMPO devices)
Baud rate 600 (9600), no parity, 1 stop, 8 data bits.
9600 in brackets means that the setting is changed by Datalyse when the device is selected.
No version command.
The command »STATUS« makes the spectrophotometer send data to the pc. For that reason this command is used in Datalyse to test the connection.
Reference measuring:
At wavelength scan the results are displayed on screen as they are being measured. When the scan is over, datalyse copies the measurings to column A and B in the table. Then you can copy column B to for instance column C using a column operation. At the next measuring the table is not erased, but new data will be written to column A and B when the scan has been completed.
Thus a wavelength scan is done in two rounds:
First measure on an empty glass and copy column B to column C. Then measure on the solution.
When both measurings have been completed, column D is calculated as B - C. Then a graph is drawn of column D as a function of the wavelength.
This is a bit troublesome but you can save the table of the reference, and start every experiment by loading a table, selecting the device and making a wavelength scan. This assumes nothing but that you measure at the same wavelengths as those saved in the table.
LBK Ultrospec II is from 1986, so it is an »old« device.
LBK spectrophotometers are distributed by:
Pharmacia Biosystems, Gydevang 21, DK-3450 Allerød, Denmark, phone: 45 16 24 00.
The new devices from Pharmacia are called Ultrospec 1000, Ultrospec 2000, Ultrospec 3000 and Ultrospec 4000.